Backbone Conformational Study of a Non-Palindromic 16 Base Pair DNA Duplex Exploring 2D31P-1H Heteronuclear Inverse Spectroscopy: Assignment of all NMR Phosphorus Resonances and Measurement of3J31P-1H3′ Coupling Constants
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چکیده
منابع مشابه
Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.
Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonabl...
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The scalar coupling constants between protons, nitrogens, and phosphorus in the metal complex chloro(triphenylphosphine)bis[bis(1-pyrazolyl)methane]ruthenium(II) chloride, [RuCl(PPh3)(BPM)2]Cl (1), were measured with a set of specially adapted NMR experiments. The absolute sign of the coupling constants was determined by relating the signs of the measured couplings to that of a one-bond proton-...
متن کاملSequence-specific assignments of the backbone 1H, 13C, and 15N resonances of the MutT enzyme by heteronuclear multidimensional NMR.
The MutT protein, a 129-residue enzyme from Escherichia coli which prevents A.T-->C.G mutations, catalyzes the hydrolysis of nucleoside triphosphates (NTP) to nucleoside monophosphates (NMP) and pyrophosphate [Bhatnagar, S. K., Bullions, L. C., & Bessman, M. J. (1991) J. Biol. Chem. 266, 9050-9054], by a mechanism involving nucleophilic substitution at the rarely attacked beta-phosphorus of NTP...
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Recently, we discovered novel silver(I)-mediated cytosine-cytosine base pair (C-Ag(I)-C) in DNA duplexes. To understand the properties of these base pairs, we searched for a DNA sequence that can be used in NMR structure determination. After extensive sequence optimizations, a non-symmetric 15-base-paired DNA duplex with a single C-Ag(I)-C base pair flanked by 14 A-T base pairs was selected. In...
متن کاملAssignment of the 31P and 1H resonances in oligonucleotides by two-dimensional NMR spectroscopy.
The use of new 1H-detected heteronuclear 1H-31P shift correlation experiments is demonstrated for oligonucleotides of 12 and 40 base pairs. The methods give unambiguous assignments of the 31P resonances and also permit identification of the C4' and C5' sugar protons. Use of the new methods enables one to make sequence-specific resonance assignments without reference to a known or assumed confor...
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ژورنال
عنوان ژورنال: Magnetic Resonance in Chemistry
سال: 1996
ISSN: 0749-1581,1097-458X
DOI: 10.1002/(sici)1097-458x(199612)34:13<s115::aid-omr66>3.0.co;2-c